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  • مهندسی ژنتیک

    سلام.میخواستم بدونم توالی ای که ژن امپیسیلین به ان متصل میشه چیه واینکه با حضور امپیسیلین چه اتفاقاتی تو محیط کشت میافته؟ممنون میشم اگه زودتر جوابمو بدین

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  • ژن مقاومت به آمپی سیلین

    The amp gene sequence was solved without consulting the protein data so that it could be determined if the DNA sequence analysis could stand alone. A distance of 789 nucleotides er from ampr gene. This with no detectable errors shows that, in fact, distance is no oblamide slab gel. The gel stacle for sequence determination. Confidence can be placed urea/50 mM Tris borate in carefully sequenced regions, even if no cross-check with 300 V. The loading of the protein is available. As a corollary, if some data about a protein, h was delayed 12 hr. The such as the composition of its tryptic fragments, is available, the top strand Hinfl site then DNA sequence may be even more easily obtained. Fewer sed were as described in than 7 mo were required both to learn all the techniques of > G. alternate G oy, C DNA sequencing and to obtain the 1100 base pairs of confirmed en run off the bottom of sequence presented here.

    ۱۳۹۰ شنبه ۲۶ آذر | 0 | ab55di

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  • amp gene sequence

    The ampr gene of Escherichia coli and other Gram-negative bacteria codes for a fl-lactamase (penicillin amido-jl-lactamhydrolase, EC 3.5.2.6) of approximately 27,000 daltons that catalyzes the hydrolysis of penicillins to penicilloic acids. Rational design of penicillins that are resistant to this penicillinase requires the elucidation of the structure of the enzyme's active site. To this end a crystallographic study of the protein is in progress,and mutations that alter the kinetic properties of the enzyme (presumably by altering the active site) have been generated .The amino acid sequence of fl-lactamase is essential to solving the structure of the enzyme. The ampr gene is easily accessible because it is carried along with tetracycline resistance on the plasmid vector pBR322. pBR322 is superior to other cloning vectors, and any information about this plasmid is likely to be useful, especially because pBR322 has been approved by the National Institutes of Health as an EK2 vector for cloning in E. coli. The plasmid RI (originally designated R7268) was isolated from the wild in London (1963) from Salmonella paratyphi B ,The ampr gene was transposed to pBR312 from RI via pSF2124 . Molecular rearrangements of pBR312 produced pBR322

    ۱۳۹۰ شنبه ۲۶ آذر | 0 | ab55di

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